Factors that contribute to the success of primary isolation of Mycoplasma genitalium from clinical samples

Abstract

Introduction. Mycoplasma genitalium, a small and slow-growing bacterium, has gained notoriety due to rapidly increasing rates of antibiotic resistance. Gap Statement. M. genitalium is difficult to culture, limiting efforts to understand its biology and the mechanisms of antimicrobial resistance. Aim. To understand factors that influence success in primary isolation of M. genitalium from clinical samples. Methodology. Neat urine or swabs (high vaginal and anal, in universal transport medium) were collected from patients with confirmed or suspected M. genitalium infections attending the Melbourne Sexual Health Centre. The specimens were stored at −80 °C prior to laboratory analysis. Initial di..